Buffer optimization for separation of bsAb in a capture step using MabSelect™ VL resin

Protein L resins bind to kappa light chain and are often used to purify antibody fragments such as dAbs and Fabs — but they can also be used to remove product-related impurities from bispecific antibodies (bsAb) at capture.

Here we study the effects of different buffer substances, mono-, di­, and tri-carboxylic acids, and concentrations on elution pH, resolution between hetero- and homodimers for bsAb’s, and removal of light chain from mAbs during elution when using MabSelect™ VL protein L resin. We’ll show the results of these experiments and discuss how buffer screening enables improvements in terms of increased elution pH and resolution.

Learn more at https://www.cytivalifesciences.com/shop/chromatography/resins/affinity-antibody/mabselect-vl-protein-l-resin-p-31823