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Key Strategies for pDNA Optimization and Scale-Up to 30 L

Unlock advanced approaches to scalable plasmid DNA (pDNA) production in this on-demand webinar, designed for CDMOs and Translational Centers seeking to enhance process efficiency and reliability. Join Denis Kole, PhD (Director, Fast Trak™ Process Development Services, Cytiva) and Charlotte Barker (Editor, BioInsights) as they present actionable insights and data-driven methodologies for optimizing pDNA workflows from bench scale to 30 L pilot scale.

Webinar highlights:

• In-depth case study detailing the transformation process, glycerol stock generation, and feasibility assessments at both 1 L and 30 L scales
• Application of a 4-factor, 2-level Design of Experiments (DoE) to systematically evaluate media types, carbon sources, feed supplementation, and vessel configurations
• Demonstration of the Xcellerex™ XDR-50 dual-purpose bioreactor’s capabilities for achieving reproducible, high-density cultures and robust pDNA titers at pilot scale
• Analysis of key results, including the impact of Terrific Broth, glycerol, and optimized feeding regimens on cell density and DNA yield
• Guidance on tech transfer, including documentation packages, batch instructions, and comprehensive reporting to support regulatory compliance and process scalability
• Real-world success stories illustrating accelerated process development, scale-up, and readiness for clinical trials

Why watch?

This webinar is tailored for scientists, engineers, and manufacturing teams who are responsible for pDNA production and process development. Discover how Cytiva’s Fast Trak™ Process Development Services can help you streamline timelines, reduce operational costs, and retain intellectual property throughout your project lifecycle. Benefit from over 35 years of Cytiva’s experience, global infrastructure, and dedicated technical support.

Ready to optimize your pDNA production process?
Watch now and transform your plasmid DNA workflow.

Learn more at: https://cytiva.link/xbg1k

Contact Cytiva: https://cytiva.link/gj57t