Can affinity capture play an important role in the removal of HMWs in biopharmaceutical process dev?

In this study, the potential of Protein A chromatography to separate antibody monomer from HMW species was investigated. A design of experiments approach was used to study the influence of four factors on the removal of HMW species: (1) elution buffer type, (2) conductivity of the elution buffer, (3) pH gradient length and (4) Protein A resin type.

During this study it was shown that HMW species can potentially be separated from antibody monomer by Protein A chromatography to the desired levels while maintaining an acceptable monomer recovery. This would result in the use of a more efficient Protein A step and allow for reduction of DSP economics.

Speaker:
Michel Eppink, Senior Director Downstream Processing, Byondis BV

Michel received his MSc. in Biology/Chemistry in 1993 from the University of Utrecht and his PhD in 1999 from the Wageningen University and Research at the Laboratory of Biochemistry with background in structure/function relationships of proteins. Michel Eppink has more than 30 years of experience in Downstream Processing and currently is Senior Director Downstream Processing at Byondis BV heading a department of 30 highly skilled coworkers developing purification/formulation processes for biopharmaceutical proteins such as monoclonal antibodies and antibody drug conjugates.

Next to that Michel Eppink is Special Professor at the Department of Bioprocess Engineering at Wageningen University and Research heading a group of PhD candidates in the research of biorefinery technologies such as new purification/analytical techniques for proteins/lipids/carbohydrates from different eukaryotic organisms (e.g. mammalian cell lines, macroalgae, microalgae, yeast). In the Netherlands Michel is a section member of the Downstream Processing section of the Dutch Biotechnology Association. Internationally he is chair of the Microalgae Bioengineering Section of the European Society of Biochemical Engineering Sciences (ESBES) and scientific committee member of the International Symposium of Proteins and Peptides Purifications (ISPPP).