Overcoming the challenges of manual tissue disaggregation

Recent technological advancements have made single-cell omics (genomics, proteomics, metabolomics) an increasingly powerful tool for diagnosis, treatment response prediction, treatment selection and monitoring, especially for cancer. The initial sample disaggregation into a single cell suspension, however, is an unestablished part of the process. Our video explains the challenges with current techniques involve manual mechanical or enzymatic disruption, which can result in losses via ruptured or attached cells, making them unsuitable for library preparation.