Asymmetric bispecific antibody purification platforms using avidity effects

In this video, we show the newly developed tools and a systematic approach that can be used to achieve high purity of the correctly paired antibody already in the capture step. The number of asymmetric bispecific antibodies have exploded in recent years. Purification of asymmetric bispecific molecules is more complicated compared to standard monoclonal antibodies. One way to purify these molecules is by using avidity effects on affinity protein A and protein L resins. We have created novel resins, without the classical Fc interaction, and we show that it is possible to allow binding to one heavy or light chain and avoid binding to the other heavy or light chain with the affinity ligands for VH3 and VL-k and by using the sequence variability in the variable part of the antibody. In this video, we show the newly developed tools and a systematic approach that can be used to achieve high purity of the correctly paired antibody already in the capture step.

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