Aix-Marseille Universite – Imaging with Typhoon™ laser scanner to observe complex activity

In this webinar you will hear about the use of Amersham Typhoon™ laser scanner imager to observe the activity of the SARS-CoV RNA polymerase protein complex.

Key takeaways:
• Visualization of the activity of the polymerase complex using primer annealed with Cy™5 fluorescent dye
• Measurement of very low concentrations (pmol) of fluorescents to measure the reaction rate for the incorporation of the annealed primer
• Visualization of NTP-analogs into the polymerase complex for potential viral therapies

Speakers

Barbara Selisko, PhD
Research Associate
Aix-Marseille University, Architecture and Function of Biological Macromolecules Laboratory (AFMB)
Barbara Selisko received her PhD in Biochemistry from the Martin-Luther-Universität in Halle, Germany. Afterwards, she worked on a variety of research positions in Germany, the UK and Mexico with a focus on protein biochemistry. Since 2000, she is a research associate at the Architecture and Fonction de Macromolécules Biologiques (AFMB) laboratory at Aix-Marseille Université. The focus of Barbara’s current research is on the functional characterization of viral replicases especially RNA-dependent RNA polymerases.

Ashleigh Shannon, PhD
Postdoctoral Researcher
Aix-Marseille University, Architecture and Function of Biological Macromolecules Laboratory (AFMB)
Ashleigh Shannon received her PhD in Biochemistry and Virology from the School of Chemistry and Molecular Biosciences at the University of Queensland Australia. The focus of her research was on the characterization of the Dengue virus NS2B-NS3 protease. Currently, she is a postdoctoral researcher at the Fonction de Macromolécules Biologiques laboratory at Aix-Marseille Université, studying the structure, function, and design of viral replicases.