In this video, Hao Huang, Product Manager for Chromatography Resins at Cytiva, shows and discusses a step-by-step high-throughput process development (HTPD) workflow to determine the optimal resin and initial conditions for hydrophobic interaction chromatography (HIC).
- The use of HTPD plates facilitates the choice of resin and initial conditions for column chromatography.
- A design of experiments (DoE) was also used to optimize the wash step.
Agenda:
1) Introduction to HIC technique
2) HTPD in process development and PreDictor™ plates
3) Background of the study
4) Step-by-step workflow in the study
- Investigation of solubility
- Screening of binding conditions and resin selection with HTPD plates
- Columns studies based on plates results (binding capacity and selectivity study)
- Screening of elution conditions with HTPD plates
- DoE optimization of wash steps in column studies
- Verification runs on columns
5) Cytiva HIC resins
